E-ISSN: 1309-0380
    Assisting in vitro fertilization by manipulating cumulus-oocyte-complexes either mechanically or enzymatically does not prevent IVF failure
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    Original Investigation
    VOLUME: 12 ISSUE: 3
    P: 135-139
    September 2011

    Assisting in vitro fertilization by manipulating cumulus-oocyte-complexes either mechanically or enzymatically does not prevent IVF failure

    J Turk Ger Gynecol Assoc 2011;12(3):135-139
    DOI: 10.5152/jtgga.2011.32
    Thomas Ebner 1
    Marianne Moser 2
    Omar Shebl 3
    Richard Mayer 4
    Gernot Tews 3
    1. Landes-Frauen- Und Kinderklinik, Ivf Abteilung, Linz, Österreich
    2. Ivf Unit, Landes-Frauen- Und Kinderklinik, Linz, Upper Austria, Austria
    3. Landes- Frauen- Und Kinderklinik, Kinderwunsch Zentrum, Linz, Austria
    4. Department Of Gynecology And Obstetrics, General Hospital Of Linz, Akh-Linz, Austria
    No information available.
    No information available
    Received Date: 17.03.2011
    Accepted Date: 10.05.2011
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    ABSTRACT

    Objective:

    This prospective study was set up in order to analyze whether additional treatment (cutting off supernumerous cumulus cells; adding hyaluronidase) of the cumulus-oocyte-complex (COC) would help to improve treatment outcome.

    Material and Methods:

    COCs from 50 patients were prospectively subdivided into a control group A (no manipulation of COC) and two study groups. In group B, surplus cumulus cells were cut off using syringes, and in the second study group COCs were incubated with a 1: 11 dilution of hyaluronidase (final concentration 7 IU/l). Main outcome measures were fertilization rate, embryo development, as well as rates of implantation, pregnancy, and live birth.

    Results:

    Fertilization was higher in group C as compared to the untreated control group A (p<0.05). However, complete fertilization failure could not be avoided by any of the modified IVF approaches. Compaction on day 4 and blastocyst quality on day 5 were significantly improved in group C as compared to group B (but not to group A). Rates of implantation, pregnancy, and live birth were not affected by any of the methods.

    Conclusion:

    ICSI seems to be the only choice for avoiding the vast majority of fertilization failures after IVF.

    Keywords: Blastocyst, cumulus-oocyte complex, fertilization, hyaluronidase, IVF

    References

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