E-ISSN: 1309-0380
    Immunohistochemical Detection And Distribution Of Cornifin Alpha And Vitamin D Receptor Expression In Vaginal Epithelium Treated With Vitamin D3
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    Clinical Study
    VOLUME: 8 ISSUE: 1
    P: 33-37
    March 2007

    Immunohistochemical Detection And Distribution Of Cornifin Alpha And Vitamin D Receptor Expression In Vaginal Epithelium Treated With Vitamin D3

    J Turk Ger Gynecol Assoc 2007;8(1):33-37
    Basak Nazan Yildirim 1
    Gülçin Abban 2
    Simin Rota 3
    Anton Jetten 4
    1. Department Of Obstetrics And Gynecology, Faculty Of Medicine, Pamukkale University, Denizli, Turkey
    2. Department Of Histology And Embryology, Faculty Of Medicine, Pamukkale University, Denizli, Turkey
    3. Department Of Biochemistery, Faculty Of Medicine, Pamukkale University, Denizli, Turkey
    4. Cell Biology Section, Laboratory Of Pulmonary Pathobiology, Nıehs, Nıh Research Triangle Park, Nc 27709, Usa
    No information available.
    No information available
    Received Date: 02.10.2006
    Accepted Date: 29.12.2006
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    ABSTRACT

    OBJECTIVE

    1, 25-dihydroxyvitamin D3 has an important role in growth and differentation of keratinizing stratified squamous epithelium. Cornifin α as a marker of vaginal epithelial differentiation distribution in vagina epithelium has not been studied. We examined distribution of VDR and cornifin α in vagina epithelium in ovariectomized and treated vit D rats

    Study design

    Bilateral ovariectomies were performed in 20 mature non-pregnant Wistar female rats. All the animals were divided into 2 groups: control group I untreated and group II i.m. 0,025µg 1,25-dihydroxyvitamin D3 injected for three days of a week for 2 weeks. In 15 days after the last injection, vaginas were removed and processed for immunohistochemistry.

    RESULTS

    Recorded epithelial differentiation, 1,25-dihydroxyvitamin D3 receptor and cornifin α expression in vaginal epithelium of control and vitamin D3 treated rats. In treated animals vaginal epithelium changed into highly- stratified keratinizing layers. 1,25-dihydroxyvitamin D3 receptor and cornifin α as a marker of squamous differentiation is present in ovariectomized rats treated with 1,25-dihydroxyvitamin D3. In contrast, cornifin α and 1,25-dihydroxyvitamin D3 receptor were absent in all layers of vaginal epithelium in control group.

    Discussion

    The results indicate that 1.25-dihydroxyvitamin D3 induced proliferation of vaginal epithelium which was consistent with the cornifin α expression. We demonstrated for the first time that 1.25-dihydroxyvitamin D3 up-regulated 1.25-dihydroxyvitamin D3 receptor expression in vaginal epithelium.

    Keywords: Vitamin D, vagina, cornifin a, VDR, immunohistochemistry

    References

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