E-ISSN: 1309-0380
HLA-E*0101 associated with recurrent spontaneous abortion
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Clinical Study
VOLUME: 8 ISSUE: 3
P: 278-284
September 2007

HLA-E*0101 associated with recurrent spontaneous abortion

J Turk Ger Gynecol Assoc 2007;8(3):278-284
Piyush Tripathi 1
Suraksha Agrawal 1
Sita Naik 2
1. Department Of Medical Genetics, Sanjay Gandhi Post Graduate Institute Of Medical Sciences, Lucknow 226014, India
2. Department Of Immunology, Sanjay Gandhi Post Graduate Institute Of Medical Sciences, Lucknow 226014, India
No information available.
No information available
Received Date: 06.11.2006
Accepted Date: 09.03.2007
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ABSTRACT

MATERIAL-METHOD:

In this study 200 recurrent spontaneous aborters (RSA) and 200 normal fertile controls were included. DNA was isolated by salting out procedure. Mutations in exon-2 and -3 were investigated by PCR-RFLP and ARMS-PCR.

RESULTS:

We have demonstrated E*0101, E*01031 and E*01032 alleles among patients and controls. Allele E*0101 was found to be associated with RSA patients (χ2 =5.214 and p=0.0224). Further HLA-E*0101 homozygotes were significantly higher among RSA patients (χ2 =9.763 and p=0.0018) as compared to controls.

CONCLUSION:

Two major alleles E*0101 and E*0103 differ in their biological and biophysical properties. HLA-E*0101 may be associated with recurrent spontaneous abortion because of decreased surface expression, lower peptide affinities and lower stability.

Keywords:
Amplification Refractory Mutation System, Human Leukocyte Antigen-E, Killer Inhibitory Receptor, Recurrent spontaneous abortion