E-ISSN: 1309-0380
Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome
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Original Investigation
VOLUME: 10 ISSUE: 3
P: 132-136
September 2009

Cytokine and nitric oxide concentrations in follicular fluid and blood serum of patients undergoing assisted reproductive treatment: relationship to outcome

J Turk Ger Gynecol Assoc 2009;10(3):132-136
Cem Fiçicioglu 1
Banu Kumbak 2
Oya Akçin 3
Rukset Attar 4
Gazi Yildirim 5
Nihan Tecellioglu 4
Narter Yesildaglar 6
1. Yeditepe University Hospital, Center For Reproductive Medicine, Head Of The Department Of Obstetrics, And Gynecology, Istanbul, Turkey
2. Istanbul Memorial Hospital, Assisted Reproduction Center, Istanbul, Turkey
3. Istanbul Memorial Hospital, Assisted Reproductive Techniques & Reproductive Genetics Centre, Istanbul, Turkey
4. Center For Reproductive Medicine, Department Of Obstetrics And Gynecology, Yeditepe University Hospital, Istanbul, Turkey
5. Department Of Gynecology And Obstetrics Medical Faculty, Yeditepe University, Istanbul, Turkey
6. Department Of Obstetrics And Gynecology, Yeditepe University School Of Medicine, Istanbul, Turkey
No information available.
No information available
Received Date: 28.04.2009
Accepted Date: 10.08.2009
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ABSTRACT

Objective:

The role of cytokines and nitric oxide (NO) in ovarian folliculogenesis and the development of mature and fertilizable oocytes is controversial. The aim of this study is to determine the concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α and NO in the follicular fluid (FF) and blood serum (S) of patients undergoing assisted reproductive treatment (ART) and to investigate whether these cytokines could be used as a predictive parameter for ART outcome.

Materials and Methods:

A retrospective clinical study was performed at a university hospital including a total of 85 women who underwent ART. FF and serum samples were collected at the time of oocyte retrieval and measured for interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α by the enzyme-linked immunosorbent assay (ELISA) technique, using commercially available kits and NO by the nitrate/nitrite colorimetric assay. The results were compared between the women who became pregnant and those who did not following ART.

Results:

No significant difference was found in the FF and blood serum concentrations of those cytokines and NO between pregnant and non-pregnant women.

Conclusion:

Follicular fluid and blood serum concentrations of IL-1β, IL-6, IL-8, IL-12, TNF-α and NO do not predict pregnancy achievement following ART.

Keywords:
assisted reproductive treatment, cytokines, follicular fluid, nitric oxide, pregnancy