E-ISSN: 1309-0380
    Association of endothelial nitric oxide synthase gene polymorphisms with endometrial carcinoma: a preliminary study
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    Original Investigation
    VOLUME: 12 ISSUE: 4
    P: 229-233
    December 2011

    Association of endothelial nitric oxide synthase gene polymorphisms with endometrial carcinoma: a preliminary study

    J Turk Ger Gynecol Assoc 2011;12(4):229-233
    DOI: 10.5152/jtgga.2011.47
    Ebru Öztürk 1
    Ebru Dikensoy 2
    Özcan Balat 3
    Mete Gürol Uğur 1
    Sibel Oğuzkan Balcı 4
    Abdullah Aydın 5
    Ülkü Kazancı 5
    Sacide Pehlivan 4
    1. Depatment Of Obstetric And Gynecology, Faculty Of Medicine, Gaziantep University, Gaziantep, Turkey
    2. Department Of Obstetrics And Gynecology, Faculty Of Medicine, Gaziantep University, Gaziantep, Turkey
    3. Gaziantep University Faculty Of Medicine Obstetric And Gyneacology Department Gaziantep,Turkey
    4. Department Of Medical Biology And Genetics, Faculty Of Medicine, Gaziantep University, Gaziantep, Turkey
    5. Department Of Pathology, Faculty Of Medicine, Gaziantep University, Gaziantep, Turkey
    No information available.
    No information available
    Received Date: 21.08.2011
    Accepted Date: 11.09.2011
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    ABSTRACT

    Objective:

    To investigate the relationship between specific endothelial nitric oxide synthase (eNOS) gene polymorphisms and endometrial cancer (ECa).

    Material and Methods:

    The study group consisted of 89 patients histologically diagnosed with the endometrioid type of endometrial carcinoma. The control group consisted of 60 randomly selected individuals who had undergone total hysterectomy. Genomic DNA was isolated from paraffin-embedded endometrial tissues. We investigated the G894T polymorphisms (G894T) and variable number tandem repeats polymorphisms in intron 4 (VNTR intron 4) in the eNOS gene by using polymerase chain reaction (PCR) and/or restriction fragment length polymorphism (RFLP). The genotype distributions and allele frequencies of the two groups were compared.

    Results:

    Analysis of the VNTR intron 4 polymorphisms in eNOS gene revealed that the frequency of the AA genotype was significantly higher in the control group, whereas the frequency of the BB genotype was significantly higher in the ECa group. Analysis of the G894T polymorphisms in eNOS gene revealed a significantly higher frequency of the GG genotype in the control group but a significantly higher frequency of the TT genotype in the endometrial cancer group.

    Conclusion:

    The G894T and VNTR intron 4 polymorphisms in eNOS gene could be an intriguing susceptibility factor that modulates an individual’s risk of ECa in the Turkish population.

    Keywords: eNOS gene polymorphisms, endometrial carcinoma

    References

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