Original Investigation

Effect of follicular fluid NO, MDA and GSH levels on in vitro fertilization outcomes


  • Ender Yalçinkaya
  • Yigit Çakiroglu
  • Emek Doger
  • Özcan Budak
  • Mustafa Çekmen
  • Eray Çaliskan

Received Date: 22.04.2013 Accepted Date: 21.05.2013 J Turk Ger Gynecol Assoc 2013;14(3):136-141 PMID: 24592092


To investigate the biochemical markers such as nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH), indicators of the oxidative status of the follicle, to predict the outcome of in vitro fertilization.

Material and Methods:

Follicular aspirates of dominant follicles were collected during oocyte retrieval. Biochemical analyses of NO, MDA and GSH were performed on all aspirates.


When the successful and unsuccessful pregnancy groups were compared in terms of NO, MDA and GSH, follicular fluid MDA was significantly higher (p=0.001) and follicular fluid NO level was significantly lower (p=0.039) in the pregnant group. Correlation analysis between oxidative stress and IVF parameters showed that MDA had a positive weak correlation with the number of grade 1 embryos (r=0.271, p=0.033) and fertilization rate (r=0.263, p=0.039). ROC curve analysis found that malondialdehyde has an area under the curve of 0.74 and can predict pregnancy with high sensitivity.


As malondialdehyde was significantly different in pregnant and non-pregnant women and had a good sensitivity profile in predicting pregnancy, it may be considered a marker for predicting IVF success.

Keywords: Follicular fluid, malondialdehyde, nitric oxide, glutathione, in vitro fertilization, pregnancy