Objective:

The role of cytokines and nitric oxide (NO) in ovarian folliculogenesis and the development of mature and fertilizable oocytes is controversial. The aim of this study is to determine the concentrations of interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α and NO in the follicular fluid (FF) and blood serum (S) of patients undergoing assisted reproductive treatment (ART) and to investigate whether these cytokines could be used as a predictive parameter for ART outcome.

Materials and Methods:

A retrospective clinical study was performed at a university hospital including a total of 85 women who underwent ART. FF and serum samples were collected at the time of oocyte retrieval and measured for interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor (TNF)-α by the enzyme-linked immunosorbent assay (ELISA) technique, using commercially available kits and NO by the nitrate/nitrite colorimetric assay. The results were compared between the women who became pregnant and those who did not following ART.

Results:

No significant difference was found in the FF and blood serum concentrations of those cytokines and NO between pregnant and non-pregnant women.

Conclusion:

Follicular fluid and blood serum concentrations of IL-1β, IL-6, IL-8, IL-12, TNF-α and NO do not predict pregnancy achievement following ART.

Keywords: assisted reproductive treatment, cytokines, follicular fluid, nitric oxide, pregnancy